Spectrophotometers


Q: What is the Beer-Lambert law?

A: The Beer-Lambert law is an equation which relates the absorption of light to the properties of the material through which the light is travelling.

The general Beer-Lambert law is usually written as:

A = E x c x L

where A is the measured absorbance, L is the path length, c is the analyte concentration and E is a constant for each species and wavelength known as the extinction coefficient.

The Beer-Lambert law is only valid at low concentration, typically <0.01M.
Q: Which model from the 73 series is right for my application?

A: The Jenway 73 series of spectrophotometers comprises of a wide range of models:-

The 7300 spectrophotometer is a non-scanning, visible wavelength instrument.
The 7305 spectrophotometer is a non-scanning, UV and visible wavelength instrument.
The 7310 spectrophotometer is a scanning, visible wavelength instrument.
The 7315 spectrophotometer is a scanning, UV and visible wavelength instrument.

In addition, Jenway offer an application specific instrument:-

The Genova spectrophotometer is a non-scanning, UV and visible wavelength instrument pre-programmed with methods and calculations for the life science industry/laboratory.


Q: What does the Z-dimension of a spectrophotometer cell/cuvette mean?

A: Z-Dimension is defined as the distance from the bottom of the cell compartment in the instrument to the centre of the light beam. The Z-dimension or beam height in the Jenway spectrophotometers is 15mm.

Q: Why is the Z-Dimension important?

A: Each instrument manufacturer optimises the height, width, focus and placement of the light beam within the design of an instrument to ensure that it performs optimally. It is important to users when attempting to use a small sample volume, as the sample will need to be placed in line with the light path.

Q: I'm having some problems printing my 6850 results on a network printer. How can I overcome this?

A: Click here to download the latest version of the Prism PC software